Mouse BNP(Brain Natriuretic Peptide) ELISA Kit

Mfr.No.

E-EL-M0204

Antigen

BNP

Alternative Names

NPPB ; GC-B ; B-Type Natriuretic Peptide ; Natriuretic Peptide Precursor B

Background

This kit uses competitive-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with Mouse BNP .During the reaction, Mouse BNP in the sample or standard competes with a fixed amount of Mouse BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse BNP Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse BNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Description

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	O.D	Average
1000	0.351 0.409	0.38
500	0.476 0.52	0.498
250	0.713 0.6830000000000001	0.698
125	0.983 1.003	0.993
62.5	1.363 1.333	1.348
31.25	1.689 1.681	1.685
15.63	1.933 1.949	1.941
0	2.287 2.303	2.295

Reactivity

Mouse

Specificity

This kit recognizes Mouse BNP in samples. No significant cross-reactivity or interference between Mouse BNP and analogues was observed.

Assay Time

2.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	4℃(shading light)
Stop Solution	1 vial, 10 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Colormetric

Detection Range

15.625--1000pg/mL

Linearity

Samples were spiked with high concentrations of Mouse BNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	84-95	98-113	95-110
1:2	Average (%)	90	103	102
1:4	Range (%)	83-99	92-106	103
1:4	Average (%)	90	98	103
1:8	Range (%)	87-101	93-106	94-108
1:8	Average (%)	92	99	100
1:16	Range (%)	88-103	90-102	97-110
1:16	Average (%)	95	97	104

Method Type

Competitive

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse BNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse BNP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	51.40	130.40	492.60	52.70	130.30	444.70
Standard deviation	3.20	7.20	22.70	2.70	7.80	17.30
C V (%)	6.23	5.52	4.61	5.12	5.99	3.89

Recovery

The recovery of Mouse BNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	88-100	95
EDTA plasma (n=5)	95-108	100
Cell culture media (n=5)	91-105	98

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

9.38 pg/mL

Uniprot	P40753

Manual

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SKU	Supplier No.	Size	Your price
BHE10302639	E-EL-M0204	96T	$495	Add to Cart
BHE10302639	E-EL-M0204	96T*5	$2227.50	Add to Cart
BHE10302639	E-EL-M0204	96T*10	$4207.50	Add to Cart
BHE10302639	E-EL-M0204	48T	$396	Add to Cart

Mouse BNP(Brain Natriuretic Peptide) ELISA Kit

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