Mouse BMP-4(Bone Morphogenetic Protein 4) CLIA Kit

Mfr.No.

E-CL-M0142

Antigen

BMP-4

Alternative Names

BMP2B ; BMP2B1 ; MCOPS6 ; ZYME ; DVR4 ; OFC11 ; ZYME

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
800	49663	55621	52642	52616
400	20819	22649	21734	21708
200	10454	8962	9708	9682
100	4250	4854	4552	4526
50	2234	2142	2188	2162
25	1062	1058	1060	1034
12.50	508	510	509	483
0	26	26	26	--

Reactivity

Mouse

Specificity

This kit recognizes Mouse BMP-4 in samples. No significant cross-reactivity or interference between Mouse BMP-4 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

12.5--800 pg/mL

Linearity

Samples were spiked with high concentrations of Mouse BMP-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	97-115	101-114	91-102
1:2	Average (%)	105	108	96
1:4	Range (%)	101-113	92-104	103-117
1:4	Average (%)	107	98	110
1:8	Range (%)	96-106	90-102	100-116
1:8	Average (%)	101	96	108
1:16	Range (%)	88-104	88-104	87-101
1:16	Average (%)	95	95	94

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse BMP-4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse BMP-4 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	43.60	71.55	312.63	47.83	78.44	304.45
Standard deviation	4.76	7.89	32.45	5.81	8.98	26.91
C V (%)	10.92	11.03	10.38	12.15	11.45	8.84

Recovery

The recovery of Mouse BMP-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	91-105	96
EDTA plasma (n=5)	100-118	107
Cell culture media (n=5)	93-108	99

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

7.5 pg/mL

Uniprot	P21275

Manual

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10301395	E-CL-M0142	96T	$682	Add to Cart
BHE10301395	E-CL-M0142	96T*5	$3069	Add to Cart
BHE10301395	E-CL-M0142	96T*10	$5797	Add to Cart
BHE10301395	E-CL-M0142	48T	$545.60	Add to Cart

Mouse BMP-4(Bone Morphogenetic Protein 4) CLIA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse BMP-4(Bone Morphogenetic Protein 4) CLIA Kit

Rate it now..!!