Mouse ATXN1(AtaXin 1) CLIA Kit

Mfr.No.

E-CL-M0122

Antigen

ATXN1

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
500	48058	55016	51537	51508
250	18928	23068	20998	20969
125	9658	9134	9396	9367
62.5	4490	4534	4512	4483
31.25	2374	2224	2299	2270
15.625	1343	1157	1250	1221
7.81	697	783	740	711
0	28	30	29	--

Specificity

This kit recognizes Mouse ATXN1 in samples. No significant cross-reactivity or interference between Mouse ATXN1 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

7.813--500 pg/mL

Linearity

Samples were spiked with high concentrations of Mouse ATXN1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	90-103	95-108	93-107
1:2	Average (%)	96	102	101
1:4	Range (%)	87-104	99-111	96-112
1:4	Average (%)	95	105	103
1:8	Range (%)	90-106	99-116	103-119
1:8	Average (%)	98	106	109
1:16	Range (%)	86-97	84-96	91-106
1:16	Average (%)	91	91	99

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse ATXN1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse ATXN1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	24.51	66.19	171.34	24.48	67.43	174.84
Standard deviation	2.38	6.00	11.70	2.45	7.41	12.05
C V (%)	9.71	9.06	6.83	10.01	10.99	6.89

Recovery

The recovery of Mouse ATXN1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	88-98	93
EDTA plasma (n=5)	87-97	92
Cell culture media (n=5)	88-100	95

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

4.69 pg/mL

Uniprot	P54254

Manual

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SKU	Supplier No.	Size	Your price
BHE10301377	E-CL-M0122	96T	$682	Add to Cart
BHE10301377	E-CL-M0122	96T*5	$3069	Add to Cart
BHE10301377	E-CL-M0122	96T*10	$5797	Add to Cart
BHE10301377	E-CL-M0122	48T	$545.60	Add to Cart

Mouse ATXN1(Ataxin 1) CLIA Kit

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