Mouse 5-LO(Arachidonate 5-LipoXygenase) CLIA Kit

Mfr.No.

E-CL-M0696

Antigen

5-LO

Alternative Names

ALOX5 ; 5-LOX ; LOX5 ; 5LPG ; LOG5

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
2000	53782	57660	55721	55693
1000	23113	24835	23974	23946
500	11408	10656	11032	11004
250	5072	5516	5294	5266
125	2648	2568	2608	2580
62.5	1368	1254	1311	1283
31.25	631	717	674	646
0	27	29	28	--

Specificity

This kit recognizes Mouse 5-LO in samples. No significant cross-reactivity or interference between Mouse 5-LO and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

31.25--2000 pg/mL

Linearity

Samples were spiked with high concentrations of Mouse 5-LO and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	98-113	88-101	98-115
1:2	Average (%)	105	95	105
1:4	Range (%)	95-111	101-118	96-111
1:4	Average (%)	102	108	102
1:8	Range (%)	93-107	96-108	84-99
1:8	Average (%)	100	103	91
1:16	Range (%)	91-104	97-114	102-118
1:16	Average (%)	96	105	108

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse 5-LO were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse 5-LO were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	105.25	310.32	873.25	95.24	338.73	822.02
Standard deviation	10.37	34.20	86.98	10.95	23.98	71.35
C V (%)	9.85	11.02	9.96	11.50	7.08	8.68

Recovery

The recovery of Mouse 5-LO spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	94-106	99
EDTA plasma (n=5)	85-97	92
Cell culture media (n=5)	90-104	95

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

18.75 pg/mL

Uniprot	P48999

Manual

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SKU	Supplier No.	Size	Your price
BHE10301872	E-CL-M0696	96T	$682	Add to Cart
BHE10301872	E-CL-M0696	96T*5	$3069	Add to Cart
BHE10301872	E-CL-M0696	96T*10	$5797	Add to Cart
BHE10301872	E-CL-M0696	48T	$545.60	Add to Cart

Mouse 5-LO(Arachidonate 5-Lipoxygenase) CLIA Kit

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