Human VEGFR-2/KDR(Vascular Endothelial Growth Factor Receptor 2) CLIA Kit

Mfr.No.

E-CL-H1031

Antigen

VEGFR-2/KDR

Alternative Names

VEGFR2 ; CD309 ; FLK1

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
1000	50856	56494	53675	53650
500	20574	24404	22489	22464
250	10622	9800	10211	10186
125	4469	5333	4901	4876
62.5	2547	2359	2453	2428
31.25	1336	1226	1281	1256
15.63	686	730	708	683
0	24	26	25	--

Reactivity

Human

Specificity

This kit recognizes Human VEGFR-2/KDR in samples. No significant cross-reactivity or interference between Human VEGFR-2/KDR and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

15.625--1000 pg/mL

Linearity

Samples were spiked with high concentrations of Human VEGFR-2/KDR and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	86-99	91-104	89-104
1:2	Average (%)	92	96	97
1:4	Range (%)	96-112	98-109	91-107
1:4	Average (%)	102	103	98
1:8	Range (%)	102-117	87-101	91-102
1:8	Average (%)	108	94	97
1:16	Range (%)	102-119	101-118	91-101
1:16	Average (%)	109	108	96

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human VEGFR-2/KDR were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human VEGFR-2/KDR were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	50.08	139.14	480.54	51.41	138.83	499.06
Standard deviation	5.13	14.86	52.62	5.07	15.05	32.89
C V (%)	10.24	10.68	10.95	9.86	10.84	6.59

Recovery

The recovery of Human VEGFR-2/KDR spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	90-104	97
EDTA plasma (n=5)	100-117	108
Cell culture media (n=5)	86-99	92

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

9.38 pg/mL

Uniprot	P35968

Manual

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SKU	Supplier No.	Size	Your price
BHE10300907	E-CL-H1031	96T	$682	Add to Cart
BHE10300907	E-CL-H1031	96T*5	$3069	Add to Cart
BHE10300907	E-CL-H1031	96T*10	$5797	Add to Cart
BHE10300907	E-CL-H1031	48T	$545.60	Add to Cart

Human VEGFR-2/KDR(Vascular Endothelial Growth Factor Receptor 2) CLIA Kit

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