Human Smad3/MADH3(Mothers Against Decapentaplegic Homolog 3) CLIA Kit

Mfr.No.

E-CL-H0868

Antigen

Smad3/MADH3

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
10	31788	33154	32471	32447
5	14896	16044	15470	15446
2.5	8004	7002	7503	7479
1.25	3573	3733	3653	3629
0.625	1805	1717	1761	1737
0.3125	888	760	824	800
0.16	348	366	357	333
0	23	25	24	--

Reactivity

Human

Specificity

This kit recognizes Human Smad3/MADH3 in samples. No significant cross-reactivity or interference between Human Smad3/MADH3 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

0.156--10 ng/mL

Linearity

Samples were spiked with high concentrations of Human Smad3/MADH3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	86-101	101-117	89-104
1:2	Average (%)	93	109	96
1:4	Range (%)	98-112	97-111	93-105
1:4	Average (%)	105	103	99
1:8	Range (%)	93-108	93-108	93-103
1:8	Average (%)	100	100	98
1:16	Range (%)	94-106	85-96	84-97
1:16	Average (%)	100	91	90

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human Smad3/MADH3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human Smad3/MADH3 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.54	1.01	3.87	0.56	1.06	4.07
Standard deviation	0.06	0.11	0.38	0.06	0.10	0.42
C V (%)	11.11	10.89	9.82	10.71	9.43	10.32

Recovery

The recovery of Human Smad3/MADH3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	94-109	101
EDTA plasma (n=5)	101-113	107
Cell culture media (n=5)	87-101	94

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

0.09 ng/mL

Uniprot	P84022

Manual

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SKU	Supplier No.	Size	Your price
BHE10300762	E-CL-H0868	96T	$682	Add to Cart
BHE10300762	E-CL-H0868	96T*5	$3069	Add to Cart
BHE10300762	E-CL-H0868	96T*10	$5797	Add to Cart
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Human Smad3/MADH3(Mothers Against Decapentaplegic Homolog 3) CLIA Kit

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