Human SCCA1(Squamous Cell Carcinoma Antigen 1) CLIA Kit

Mfr.No.

E-CL-H1081

Antigen

SCCA1

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
1000	49276	58074	53675	53650
500	22270	22708	22489	22464
250	10812	9610	10211	10186
125	4775	5027	4901	4876
62.5	2575	2331	2453	2428
31.25	1368	1194	1281	1256
15.63	707	709	708	683
0	24	26	25	--

Reactivity

Human

Specificity

This kit recognizes Human SCCA1 in samples. No significant cross-reactivity or interference between Human SCCA1 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

15.625--1000 pg/mL

Linearity

Samples were spiked with high concentrations of Human SCCA1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	104-119	87-99	91-107
1:2	Average (%)	109	92	98
1:4	Range (%)	88-101	98-112	88-99
1:4	Average (%)	93	104	93
1:8	Range (%)	91-106	94-106	85-100
1:8	Average (%)	99	100	91
1:16	Range (%)	95-108	90-102	103-119
1:16	Average (%)	102	97	109

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human SCCA1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human SCCA1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	47.27	109.41	423.38	47.67	101.57	458.79
Standard deviation	5.65	10.89	41.11	4.60	7.94	43.26
C V (%)	11.95	9.95	9.71	9.65	7.82	9.43

Recovery

The recovery of Human SCCA1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	94-112	102
EDTA plasma (n=5)	86-98	93
Cell culture media (n=5)	91-103	96

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

9.38 pg/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10300954	E-CL-H1081	96T	$682	Add to Cart
BHE10300954	E-CL-H1081	96T*5	$3069	Add to Cart
BHE10300954	E-CL-H1081	96T*10	$5797	Add to Cart
BHE10300954	E-CL-H1081	48T	$545.60	Add to Cart

Human SCCA1(Squamous Cell Carcinoma Antigen 1) CLIA Kit

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