Human REG3gamma(Regenerating Islet DerIVed Protein 3 Gamma) CLIA Kit

Mfr.No.

E-CL-H0839

Antigen

REG3γ

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
20	33657	34307	33982	33950
10	12263	12677	12470	12438
5	5264	5236	5250	5218
2.5	2382	2666	2524	2492
1.25	1419	1347	1383	1351
0.625	912	822	867	835
0.31	600	646	623	591
0	32	32	32	--

Specificity

This kit recognizes Human REG3γ in samples. No significant cross-reactivity or interference between Human REG3γ and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

0.313--20 ng/mL

Linearity

Samples were spiked with high concentrations of Human REG3γ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	90-104	101-115	88-101
1:2	Average (%)	97	107	93
1:4	Range (%)	86-100	84-96	93-108
1:4	Average (%)	93	91	100
1:8	Range (%)	103-116	101-118	95-109
1:8	Average (%)	110	108	103
1:16	Range (%)	98-113	92-106	97-112
1:16	Average (%)	104	100	104

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human REG3γ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human REG3γ were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.01	2.28	7.09	0.95	2.26	6.60
Standard deviation	0.12	0.19	0.46	0.08	0.20	0.48
C V (%)	11.88	8.33	6.49	8.42	8.85	7.27

Recovery

The recovery of Human REG3γ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	88-101	93
EDTA plasma (n=5)	97-111	105
Cell culture media (n=5)	93-104	99

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

0.19 ng/mL

Uniprot	Q6UW15

Manual

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SKU	Supplier No.	Size	Your price
BHE10300736	E-CL-H0839	96T	$682	Add to Cart
BHE10300736	E-CL-H0839	96T*5	$3069	Add to Cart
BHE10300736	E-CL-H0839	96T*10	$5797	Add to Cart
BHE10300736	E-CL-H0839	48T	$545.60	Add to Cart

Human REG3gamma(Regenerating Islet Derived Protein 3 Gamma) CLIA Kit

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