Human ProGRP(Pro-Gastrin Releasing Peptide) CLIA Kit

Mfr.No.

E-CL-H1238

Antigen

ProGRP

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
1000	52381	54969	53675	53650
500	21579	23399	22489	22464
250	10853	9569	10211	10186
125	4848	4954	4901	4876
62.5	2510	2396	2453	2428
31.25	1310	1252	1281	1256
15.63	663	753	708	683
0	25	25	25	--

Specificity

This kit recognizes Human ProGRP in samples. No significant cross-reactivity or interference between Human ProGRP and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

15.625--1000 pg/mL

Linearity

Samples were spiked with high concentrations of Human ProGRP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	100-116	91-106	96-111
1:2	Average (%)	108	97	103
1:4	Range (%)	89-104	102-116	86-98
1:4	Average (%)	96	110	92
1:8	Range (%)	102-113	101-116	86-99
1:8	Average (%)	107	108	93
1:16	Range (%)	93-108	91-104	96-112
1:16	Average (%)	100	99	102

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ProGRP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ProGRP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	53.31	117.63	490.94	49.31	105.93	493.53
Standard deviation	6.53	12.41	52.19	5.72	9.24	34.40
C V (%)	12.25	10.55	10.63	11.60	8.72	6.97

Recovery

The recovery of Human ProGRP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	93-106	99
EDTA plasma (n=5)	88-98	93
Cell culture media (n=5)	96-110	103

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

9.38 pg/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10301089	E-CL-H1238	96T	$682	Add to Cart
BHE10301089	E-CL-H1238	96T*5	$3069	Add to Cart
BHE10301089	E-CL-H1238	96T*10	$5797	Add to Cart
BHE10301089	E-CL-H1238	48T	$545.60	Add to Cart

Human ProGRP(Pro-Gastrin Releasing Peptide) CLIA Kit

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