Human NOS1/nNOS(Nitric OXide Synthase 1, Neuronal) CLIA Kit

Mfr.No.

E-CL-H0537

Antigen

NOS1/nNOS

Alternative Names

bNOS ; IHPS1

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
2000	51108	60334	55721	55693
1000	23725	24223	23974	23946
500	11185	10879	11032	11004
250	5098	5490	5294	5266
125	2629	2587	2608	2580
62.5	1347	1275	1311	1283
31.25	650	698	674	646
0	27	29	28	--

Specificity

This kit recognizes Human NOS1/nNOS in samples. No significant cross-reactivity or interference between Human NOS1/nNOS and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

31.25--2000 pg/mL

Linearity

Samples were spiked with high concentrations of Human NOS1/nNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	96-109	99-110	88-100
1:2	Average (%)	103	104	93
1:4	Range (%)	98-114	91-106	97-110
1:4	Average (%)	105	99	104
1:8	Range (%)	88-100	97-110	101-115
1:8	Average (%)	94	104	107
1:16	Range (%)	96-113	98-115	89-104
1:16	Average (%)	103	105	96

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human NOS1/nNOS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human NOS1/nNOS were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	91.67	303.12	894.45	83.50	310.25	808.92
Standard deviation	9.56	25.73	62.16	9.83	33.94	62.45
C V (%)	10.43	8.49	6.95	11.77	10.94	7.72

Recovery

The recovery of Human NOS1/nNOS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	90-104	96
EDTA plasma (n=5)	95-106	101
Cell culture media (n=5)	83-98	90

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

18.75 pg/mL

Uniprot	P29475

Manual

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SKU	Supplier No.	Size	Your price
BHE10300465	E-CL-H0537	96T	$682	Add to Cart
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BHE10300465	E-CL-H0537	96T*10	$5797	Add to Cart
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Human NOS1/nNOS(Nitric Oxide Synthase 1, Neuronal) CLIA Kit

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