Human MRC2(Mannose Receptor C Type 2) CLIA Kit

Mfr.No.

E-CL-H1396

Antigen

MRC2

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
4000	55981	62181	59081	59046
2000	26662	29056	27859	27824
1000	14156	12812	13484	13449
500	6287	6923	6605	6570
250	3439	3047	3243	3208
125	1688	1474	1581	1546
62.50	740	770	755	720
0	34	36	35	--

Specificity

This kit recognizes Human MRC2 in samples. No significant cross-reactivity or interference between Human MRC2 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

62.5--4000 pg/mL

Linearity

Samples were spiked with high concentrations of Human MRC2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	95-107	86-102	94-108
1:2	Average (%)	101	93	102
1:4	Range (%)	94-107	102-115	95-109
1:4	Average (%)	102	108	101
1:8	Range (%)	85-96	92-104	91-103
1:8	Average (%)	91	99	97
1:16	Range (%)	88-99	89-102	84-99
1:16	Average (%)	94	96	91

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MRC2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MRC2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	207.98	480.52	1431.39	212.36	457.99	1359.01
Standard deviation	24.56	44.83	113.37	22.28	40.72	130.33
C V (%)	11.81	9.33	7.92	10.49	8.89	9.59

Recovery

The recovery of Human MRC2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	93-105	99
EDTA plasma (n=5)	87-100	94
Cell culture media (n=5)	90-104	97

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

37.5 pg/mL

Uniprot	Q9UBG0

Manual

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SKU	Supplier No.	Size	Your price
BHE10301226	E-CL-H1396	96T	$682	Add to Cart
BHE10301226	E-CL-H1396	96T*5	$3069	Add to Cart
BHE10301226	E-CL-H1396	96T*10	$5797	Add to Cart
BHE10301226	E-CL-H1396	48T	$545.60	Add to Cart

Human MRC2(Mannose Receptor C Type 2) CLIA Kit

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