Human IGFALS(Insulin Like Growth Factor Binding Protein, Acid Labile Subunit) CLIA Kit

Mfr.No.

E-CL-H0360

Antigen

IGFALS

Alternative Names

ALS

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
20	32004	35960	33982	33950
10	11899	13041	12470	12438
5	5319	5181	5250	5218
2.5	2491	2557	2524	2492
1.25	1489	1277	1383	1351
0.625	877	857	867	835
0.31	595	651	623	591
0	32	32	32	--

Specificity

This kit recognizes Human IGFALS in samples. No significant cross-reactivity or interference between Human IGFALS and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

0.313--20 ng/mL

Linearity

Samples were spiked with high concentrations of Human IGFALS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	90-102	92-105	91-104
1:2	Average (%)	95	99	98
1:4	Range (%)	102-119	98-114	86-98
1:4	Average (%)	109	104	92
1:8	Range (%)	87-98	99-116	90-105
1:8	Average (%)	93	107	97
1:16	Range (%)	85-96	101-113	88-103
1:16	Average (%)	90	106	94

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IGFALS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IGFALS were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.00	3.15	9.19	0.90	3.34	8.60
Standard deviation	0.13	0.28	1.02	0.08	0.36	0.93
C V (%)	13.00	8.89	11.10	8.89	10.78	10.81

Recovery

The recovery of Human IGFALS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	88-104	95
EDTA plasma (n=5)	100-115	108
Cell culture media (n=5)	85-96	91

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

0.19 ng/mL

Uniprot	P35858

Manual

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SKU	Supplier No.	Size	Your price
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Human IGFALS(Insulin Like Growth Factor Binding Protein, Acid Labile Subunit) CLIA Kit

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