Human HSP-90(Heat Shock Protein 90) CLIA Kit

Mfr.No.

E-CL-H1154

Antigen

HSP-90

Alternative Names

Hsp90

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
4000	54348	63814	59081	59046
2000	25749	29969	27859	27824
1000	13655	13313	13484	13449
500	6426	6784	6605	6570
250	3400	3086	3243	3208
125	1627	1535	1581	1546
62.50	755	755	755	720
0	35	35	35	--

Specificity

This kit recognizes Human HSP-90 in samples. No significant cross-reactivity or interference between Human HSP-90 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

62.5--4000 pg/mL

Linearity

Samples were spiked with high concentrations of Human HSP-90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	98-113	86-98	94-109
1:2	Average (%)	104	92	100
1:4	Range (%)	99-111	99-115	99-114
1:4	Average (%)	106	105	105
1:8	Range (%)	96-107	101-117	100-119
1:8	Average (%)	101	109	108
1:16	Range (%)	96-111	83-99	90-106
1:16	Average (%)	102	90	98

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human HSP-90 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human HSP-90 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	222.05	377.85	1698.93	237.82	375.25	1576.39
Standard deviation	23.32	35.33	156.30	22.14	43.42	166.15
C V (%)	10.50	9.35	9.20	9.31	11.57	10.54

Recovery

The recovery of Human HSP-90 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	91-103	97
EDTA plasma (n=5)	93-107	100
Cell culture media (n=5)	100-117	108

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

37.5 pg/mL

Uniprot	P08238

Manual

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SKU	Supplier No.	Size	Your price
BHE10301021	E-CL-H1154	96T	$682	Add to Cart
BHE10301021	E-CL-H1154	96T*5	$3069	Add to Cart
BHE10301021	E-CL-H1154	96T*10	$5797	Add to Cart
BHE10301021	E-CL-H1154	48T	$545.60	Add to Cart

Human HSP-90(Heat Shock Protein 90) CLIA Kit

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