Human GTF2A1(General Transcription Factor IIA, Polypeptide 1) CLIA Kit

Mfr.No.

E-CL-H0326

Antigen

GTF2A1

Alternative Names

TF2A1 ; TFIIA ; TFIIA-42 ; TFIIAL

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
5000	61146	61366	61256	61233
2500	31364	37344	34354	34331
1250	18814	17524	18169	18146
625	8696	10088	9392	9369
312.5	5049	4617	4833	4810
156.25	2682	2340	2511	2488
78.13	1258	1420	1339	1316
0	22	24	23	--

Specificity

This kit recognizes Human GTF2A1 in samples. No significant cross-reactivity or interference between Human GTF2A1 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

78.125--5000 pg/mL

Linearity

Samples were spiked with high concentrations of Human GTF2A1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	95-109	93-106	88-101
1:2	Average (%)	101	100	94
1:4	Range (%)	95-110	92-104	101-117
1:4	Average (%)	102	98	108
1:8	Range (%)	99-111	102-118	99-109
1:8	Average (%)	104	109	104
1:16	Range (%)	102-116	90-105	89-99
1:16	Average (%)	108	96	94

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human GTF2A1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human GTF2A1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	276.73	541.51	2290.84	294.81	591.43	2151.34
Standard deviation	26.73	54.58	175.48	36.26	43.06	212.98
C V (%)	9.66	10.08	7.66	12.30	7.28	9.90

Recovery

The recovery of Human GTF2A1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	91-105	97
EDTA plasma (n=5)	99-119	108
Cell culture media (n=5)	97-111	105

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

46.88 pg/mL

Uniprot	P52655

Manual

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SKU	Supplier No.	Size	Your price
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Human GTF2A1(General Transcription Factor IIA, Polypeptide 1) CLIA Kit

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