Human GSTpi(Glutathione S Transferases Pi) CLIA Kit

Mfr.No.

E-CL-H0879

Antigen

GSTpi

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
30	32005	39013	35509	35469
15	12486	14508	13497	13457
7.5	5822	5814	5818	5778
3.75	2642	2978	2810	2770
1.875	1566	1462	1514	1474
0.9375	937	899	918	878
0.47	599	667	633	593
0	38	42	40	--

Reactivity

Human

Specificity

This kit recognizes Human GSTpi in samples. No significant cross-reactivity or interference between Human GSTpi and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

0.469--30 ng/mL

Linearity

Samples were spiked with high concentrations of Human GSTpi and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	87-98	101-117	94-110
1:2	Average (%)	93	107	101
1:4	Range (%)	96-110	94-106	85-100
1:4	Average (%)	103	101	91
1:8	Range (%)	83-95	85-99	98-115
1:8	Average (%)	90	92	105
1:16	Range (%)	87-97	95-108	87-102
1:16	Average (%)	92	100	93

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human GSTpi were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human GSTpi were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.62	3.83	12.77	1.50	4.00	12.01
Standard deviation	0.18	0.44	1.36	0.18	0.40	1.29
C V (%)	11.11	11.49	10.65	12.00	10.00	10.74

Recovery

The recovery of Human GSTpi spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	87-103	95
EDTA plasma (n=5)	100-111	105
Cell culture media (n=5)	100-115	106

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

0.28 ng/mL

Uniprot	P09211

Manual

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10300772	E-CL-H0879	96T	$682	Add to Cart
BHE10300772	E-CL-H0879	96T*5	$3069	Add to Cart
BHE10300772	E-CL-H0879	96T*10	$5797	Add to Cart
BHE10300772	E-CL-H0879	48T	$545.60	Add to Cart

Human GSTpi(Glutathione S Transferases Pi) CLIA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human GSTpi(Glutathione S Transferases Pi) CLIA Kit

Rate it now..!!