Human FcalphaR/CD89(Receptor I for the Fc Fragment of IgA) CLIA Kit

Mfr.No.

E-CL-H1088

Antigen

FcαR/CD89

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
10	30695	34247	32471	32447
5	14763	16177	15470	15446
2.5	7506	7500	7503	7479
1.25	3581	3725	3653	3629
0.625	1816	1706	1761	1737
0.3125	854	794	824	800
0.16	342	372	357	333
0	23	25	24	--

Specificity

This kit recognizes Human FcαR/CD89 in samples. No significant cross-reactivity or interference between Human FcαR/CD89 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

0.156--10 ng/mL

Linearity

Samples were spiked with high concentrations of Human FcαR/CD89 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	90-105	93-108	90-102
1:2	Average (%)	96	101	97
1:4	Range (%)	89-102	90-103	84-98
1:4	Average (%)	96	96	91
1:8	Range (%)	99-115	91-106	89-101
1:8	Average (%)	106	98	94
1:16	Range (%)	87-100	99-114	87-97
1:16	Average (%)	92	106	92

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human FcαR/CD89 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human FcαR/CD89 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.46	0.86	4.21	0.46	0.81	3.85
Standard deviation	0.05	0.09	0.47	0.05	0.07	0.41
C V (%)	10.87	10.47	11.16	10.87	8.64	10.65

Recovery

The recovery of Human FcαR/CD89 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	99-114	106
EDTA plasma (n=5)	97-111	105
Cell culture media (n=5)	95-108	101

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

0.09 ng/mL

Uniprot	P24071

Manual

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SKU	Supplier No.	Size	Your price
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BHE10300960	E-CL-H1088	96T*10	$5797	Add to Cart
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Human FcalphaR/CD89(Receptor I for the Fc Fragment of IgA) CLIA Kit

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