Human F13A1(Coagulation Factor xIII A1 Polypeptide) CLIA Kit

Mfr.No.

E-CL-H0551

Antigen

F13A1

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
800	50662	54622	52642	52616
400	20267	23201	21734	21708
200	10518	8898	9708	9682
100	4357	4747	4552	4526
50	2359	2017	2188	2162
25	1090	1030	1060	1034
12.50	506	512	509	483
0	25	27	26	--

Reactivity

Human

Specificity

This kit recognizes Human F13A1 in samples. No significant cross-reactivity or interference between Human F13A1 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

12.5--800 pg/mL

Linearity

Samples were spiked with high concentrations of Human F13A1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	98-110	96-110	98-113
1:2	Average (%)	104	101	103
1:4	Range (%)	98-115	100-115	85-99
1:4	Average (%)	106	106	91
1:8	Range (%)	102-119	100-116	102-118
1:8	Average (%)	109	106	108
1:16	Range (%)	85-95	100-116	99-112
1:16	Average (%)	90	109	106

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human F13A1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human F13A1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	37.66	72.96	305.26	40.70	73.29	277.75
Standard deviation	4.56	7.30	34.40	3.51	6.63	23.64
C V (%)	12.11	10.01	11.27	8.62	9.05	8.51

Recovery

The recovery of Human F13A1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	86-99	92
EDTA plasma (n=5)	91-103	98
Cell culture media (n=5)	85-97	91

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

7.5 pg/mL

Uniprot	P00488

Manual

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SKU	Supplier No.	Size	Your price
BHE10300478	E-CL-H0551	96T	$682	Add to Cart
BHE10300478	E-CL-H0551	96T*5	$3069	Add to Cart
BHE10300478	E-CL-H0551	96T*10	$5797	Add to Cart
BHE10300478	E-CL-H0551	48T	$545.60	Add to Cart

Human F13A1(Coagulation Factor XIII A1 Polypeptide) CLIA Kit

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