Human F10(Coagulation Factor X) CLIA Kit

Mfr.No.

E-CL-H0547

Antigen

F10

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
2000	53105	58337	55721	55693
1000	23446	24502	23974	23946
500	11435	10629	11032	11004
250	4998	5590	5294	5266
125	2694	2522	2608	2580
62.5	1364	1258	1311	1283
31.25	672	676	674	646
0	27	29	28	--

Reactivity

Human

Specificity

This kit recognizes Human F10 in samples. No significant cross-reactivity or interference between Human F10 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

31.25--2000 pg/mL

Linearity

Samples were spiked with high concentrations of Human F10 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	89-101	93-105	99-111
1:2	Average (%)	96	99	105
1:4	Range (%)	96-109	84-99	94-106
1:4	Average (%)	101	91	100
1:8	Range (%)	94-106	95-111	94-110
1:8	Average (%)	100	102	101
1:16	Range (%)	103-118	90-104	94-106
1:16	Average (%)	109	97	99

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human F10 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human F10 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	92.75	173.18	751.08	97.56	182.93	759.08
Standard deviation	7.69	19.90	78.64	9.23	17.73	74.85
C V (%)	8.29	11.49	10.47	9.46	9.69	9.86

Recovery

The recovery of Human F10 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	86-96	91
EDTA plasma (n=5)	85-97	91
Cell culture media (n=5)	86-99	92

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

18.75 pg/mL

Uniprot	P00742

Manual

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10300474	E-CL-H0547	96T	$682	Add to Cart
BHE10300474	E-CL-H0547	96T*5	$3069	Add to Cart
BHE10300474	E-CL-H0547	96T*10	$5797	Add to Cart
BHE10300474	E-CL-H0547	48T	$545.60	Add to Cart

Human F10(Coagulation Factor X) CLIA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human F10(Coagulation Factor X) CLIA Kit

Rate it now..!!