Human CSF2Rbeta(Colony Stimulating Factor 2 Receptor Beta) CLIA Kit

Mfr.No.

E-CL-H0293

Antigen

CSF2Rβ

Alternative Names

CSF2RB ; IL3RB ; CD131

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
2000	53990	57452	55721	55693
1000	22434	25514	23974	23946
500	11690	10374	11032	11004
250	5203	5385	5294	5266
125	2678	2538	2608	2580
62.5	1410	1212	1311	1283
31.25	672	676	674	646
0	27	29	28	--

Specificity

This kit recognizes Human CSF2Rβ in samples. No significant cross-reactivity or interference between Human CSF2Rβ and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

31.25--2000 pg/mL

Linearity

Samples were spiked with high concentrations of Human CSF2Rβ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	97-109	85-97	92-104
1:2	Average (%)	102	91	98
1:4	Range (%)	88-100	96-113	97-110
1:4	Average (%)	95	104	104
1:8	Range (%)	93-104	95-106	89-101
1:8	Average (%)	98	101	94
1:16	Range (%)	93-106	90-104	97-111
1:16	Average (%)	100	98	102

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CSF2Rβ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CSF2Rβ were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	93.63	211.51	811.64	87.71	195.80	837.74
Standard deviation	11.33	20.37	63.31	11.18	18.15	58.39
C V (%)	12.10	9.63	7.80	12.75	9.27	6.97

Recovery

The recovery of Human CSF2Rβ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	98-115	106
EDTA plasma (n=5)	94-108	101
Cell culture media (n=5)	93-106	98

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

18.75 pg/mL

Manual

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10300256	E-CL-H0293	96T	$682	Add to Cart
BHE10300256	E-CL-H0293	96T*5	$3069	Add to Cart
BHE10300256	E-CL-H0293	96T*10	$5797	Add to Cart
BHE10300256	E-CL-H0293	48T	$545.60	Add to Cart

Human CSF2Rbeta(Colony Stimulating Factor 2 Receptor Beta) CLIA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human CSF2Rbeta(Colony Stimulating Factor 2 Receptor Beta) CLIA Kit

Rate it now..!!