Human COL1alpha2(Collagen Type I Alpha 2) CLIA Kit

Mfr.No.

E-CL-H0556

Antigen

COL1α2

Alternative Names

COL1A2 ; OI4

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
2000	52296	59146	55721	55693
1000	21609	26339	23974	23946
500	11072	10992	11032	11004
250	5101	5487	5294	5266
125	2810	2406	2608	2580
62.5	1402	1220	1311	1283
31.25	673	675	674	646
0	27	29	28	--

Specificity

This kit recognizes Human COL1α2 in samples. No significant cross-reactivity or interference between Human COL1α2 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

31.25--2000 pg/mL

Linearity

Samples were spiked with high concentrations of Human COL1α2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	88-101	101-114	100-115
1:2	Average (%)	93	108	106
1:4	Range (%)	88-101	87-98	100-114
1:4	Average (%)	95	92	106
1:8	Range (%)	94-107	103-115	87-104
1:8	Average (%)	101	109	95
1:16	Range (%)	93-109	100-113	92-104
1:16	Average (%)	100	105	98

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human COL1α2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human COL1α2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	103.56	189.05	753.17	109.77	198.00	744.85
Standard deviation	12.43	16.07	48.73	12.43	14.59	64.36
C V (%)	12.00	8.50	6.47	11.32	7.37	8.64

Recovery

The recovery of Human COL1α2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	99-114	107
EDTA plasma (n=5)	86-96	91
Cell culture media (n=5)	89-101	94

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

18.75 pg/mL

Uniprot	P08123

Manual

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SKU	Supplier No.	Size	Your price
BHE10300483	E-CL-H0556	96T	$682	Add to Cart
BHE10300483	E-CL-H0556	96T*5	$3069	Add to Cart
BHE10300483	E-CL-H0556	96T*10	$5797	Add to Cart
BHE10300483	E-CL-H0556	48T	$545.60	Add to Cart

Human COL1alpha2(Collagen Type I Alpha 2) CLIA Kit

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