Human CK-19/KRT19(Cytokeratin 19) CLIA Kit

Mfr.No.

E-CL-H1245

Antigen

CK-19/KRT19

Alternative Names

CK19 ; K19 ; Keratin 19 ; K1CS ; Keratin ; type I cytoskeletal 19 ; 40-kDa Keratin Intermediate Filament

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
1000	50041	57309	53675	53650
500	21066	23912	22489	22464
250	10464	9958	10211	10186
125	4705	5097	4901	4876
62.5	2633	2273	2453	2428
31.25	1343	1219	1281	1256
15.63	675	741	708	683
0	25	25	25	--

Specificity

This kit recognizes Human CK-19/KRT19 in samples. No significant cross-reactivity or interference between Human CK-19/KRT19 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

15.625--1000 pg/mL

Linearity

Samples were spiked with high concentrations of Human CK-19/KRT19 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	100-114	84-96	83-95
1:2	Average (%)	106	91	90
1:4	Range (%)	91-102	85-97	99-115
1:4	Average (%)	97	91	106
1:8	Range (%)	89-99	90-103	103-118
1:8	Average (%)	94	97	110
1:16	Range (%)	100-115	95-108	102-114
1:16	Average (%)	107	101	108

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CK-19/KRT19 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CK-19/KRT19 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	48.34	158.97	387.58	48.15	148.47	406.72
Standard deviation	5.81	12.22	37.83	4.93	15.74	41.24
C V (%)	12.02	7.69	9.76	10.24	10.60	10.14

Recovery

The recovery of Human CK-19/KRT19 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	96-107	102
EDTA plasma (n=5)	90-106	97
Cell culture media (n=5)	89-100	95

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

9.38 pg/mL

Uniprot	P08727

Manual

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SKU	Supplier No.	Size	Your price
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Human CK-19/KRT19(Cytokeratin 19) CLIA Kit

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