Human CA3(Carbonic Anhydrase III, Muscle Specific) CLIA Kit

Mfr.No.

E-CL-H0294

Antigen

CA3

Alternative Names

CAIII ; CA-III ; Car3

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
40	34122	38954	36538	36508
20	13225	15407	14316	14286
10	6766	5780	6273	6243
5	2946	3090	3018	2988
2.5	1613	1551	1582	1552
1.25	982	842	912	882
0.63	554	624	589	559
0	29	31	30	--

Specificity

This kit recognizes Human CA3 in samples. No significant cross-reactivity or interference between Human CA3 and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

0.625--40 ng/mL

Linearity

Samples were spiked with high concentrations of Human CA3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	95-110	92-106	90-102
1:2	Average (%)	102	98	97
1:4	Range (%)	83-99	88-103	91-103
1:4	Average (%)	90	94	96
1:8	Range (%)	100-114	101-117	92-107
1:8	Average (%)	105	107	100
1:16	Range (%)	99-115	99-113	85-99
1:16	Average (%)	106	107	91

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CA3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CA3 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.95	3.50	17.49	1.85	3.63	18.82
Standard deviation	0.17	0.26	1.81	0.16	0.29	1.50
C V (%)	8.72	7.43	10.35	8.65	7.99	7.97

Recovery

The recovery of Human CA3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	99-114	105
EDTA plasma (n=5)	99-115	106
Cell culture media (n=5)	101-116	107

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

0.38 ng/mL

Uniprot	P07451

Manual

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SKU	Supplier No.	Size	Your price
BHE10300257	E-CL-H0294	96T	$682	Add to Cart
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BHE10300257	E-CL-H0294	96T*10	$5797	Add to Cart
BHE10300257	E-CL-H0294	48T	$545.60	Add to Cart

Human CA3(Carbonic Anhydrase III, Muscle Specific) CLIA Kit

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