Human C4b(Complement Component 4b) CLIA Kit

Mfr.No.

E-CL-H0575

Antigen

C4B

Alternative Names

C4-B ; CPAMD3 ; C4 (Chido Blood Group) ; Basic C4

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
60	33527	40799	37163	37138
30	15383	16575	15979	15954
15	7931	6811	7371	7346
7.5	3335	3791	3563	3538
3.75	1867	1699	1783	1758
1.875	941	907	924	899
0.94	483	521	502	477
0	25	25	25	--

Specificity

This kit recognizes Human C4b in samples. No significant cross-reactivity or interference between Human C4b and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

0.938--60 ng/mL

Linearity

Samples were spiked with high concentrations of Human C4b and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	93-110	86-97	85-98
1:2	Average (%)	100	91	92
1:4	Range (%)	98-110	84-97	100-113
1:4	Average (%)	104	91	107
1:8	Range (%)	98-111	101-115	86-102
1:8	Average (%)	104	107	93
1:16	Range (%)	102-114	101-117	98-112
1:16	Average (%)	108	110	105

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human C4b were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human C4b were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	3.24	9.00	23.86	3.29	8.95	22.48
Standard deviation	0.33	0.93	1.79	0.39	0.91	1.66
C V (%)	10.19	10.33	7.50	11.85	10.17	7.38

Recovery

The recovery of Human C4b spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	89-105	96
EDTA plasma (n=5)	85-95	90
Cell culture media (n=5)	86-100	92

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

0.56 ng/mL

Uniprot	P0C0L5

Manual

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SKU	Supplier No.	Size	Your price
BHE10300501	E-CL-H0575	96T	$682	Add to Cart
BHE10300501	E-CL-H0575	96T*5	$3069	Add to Cart
BHE10300501	E-CL-H0575	96T*10	$5797	Add to Cart
BHE10300501	E-CL-H0575	48T	$545.60	Add to Cart

Human C4b(Complement Component 4b) CLIA Kit

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