Human BMG/beta2-MG(Beta-2-Microglobulin) ELISA Kit

Mfr.No.

E-EL-H2188

Antigen

BMG/β2-MG

Alternative Names

B2MG ; B2M

Background

This kit uses competitive-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with Human BMG/β2-MG .During the reaction, Human BMG/β2-MG in the sample or standard competes with a fixed amount of Human BMG/β2-MG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human BMG/β2-MG Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human BMG/β2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

Description

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	O.D	Average
2000	0.312 0.35	0.331
1000	0.397 0.453	0.425
500	0.614 0.574	0.594
250	0.846 0.886	0.866
125	1.228 1.222	1.225
62.5	1.605 1.583	1.594
31.25	1.882 1.896	1.889
0	2.297 2.301	2.299

Reactivity

Human

Specificity

This kit recognizes Human BMG/β2-MG in samples. No significant cross-reactivity or interference between Human BMG/β2-MG and analogues was observed.

Assay Time

2.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	4℃(shading light)
Stop Solution	1 vial, 10 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Colormetric

Detection Range

31.25--2000ng/mL

Linearity

Samples were spiked with high concentrations of Human BMG/β2-MG and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	100-114	92-107	99-110
1:2	Average (%)	106	98	104
1:4	Range (%)	86-97	87-102	102
1:4	Average (%)	91	94	102
1:8	Range (%)	88-103	95-110	98-114
1:8	Average (%)	94	100	106
1:16	Range (%)	90-103	87-103	100-116
1:16	Average (%)	95	94	106

Method Type

Competitive

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human BMG/β2-MG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human BMG/β2-MG were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	96.60	219.60	946.40	87.00	201.80	868.10
Standard deviation	6.40	11.20	35.00	5.60	10.10	35.60
C V (%)	6.63	5.10	3.70	6.44	5.00	4.10

Recovery

The recovery of Human BMG/β2-MG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	93-108	101
EDTA plasma (n=5)	85-97	91
Cell culture media (n=5)	95-110	101

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

18.75 ng/mL

Uniprot	P61769

Manual

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SKU	Supplier No.	Size	Your price
BHE10302616	E-EL-H2188	96T	$609	Add to Cart
BHE10302616	E-EL-H2188	96T*5	$2740.50	Add to Cart
BHE10302616	E-EL-H2188	96T*10	$5176.50	Add to Cart
BHE10302616	E-EL-H2188	48T	$487.20	Add to Cart

Human BMG/beta2-MG(Beta-2-Microglobulin) ELISA Kit

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