Human AP13(Apelin 13) ELISA Kit

Mfr.No.

E-EL-H0458

Antigen

AP13

Alternative Names

AP13

Background

This kit uses competitive-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with Human AP13 .During the reaction, Human AP13 in the sample or standard competes with a fixed amount of Human AP13 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human AP13 Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human AP13 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	O.D	Average
8000	0.4 0.412	0.406
4000	0.482 0.534	0.508
2000	0.7 0.6899999999999999	0.6950000000000001
1000	0.988 1.012	1
500	1.43 1.41	1.42
250	1.887 1.871	1.879
125.00	2.263 2.277	2.27
0	2.862 2.878	2.87

Specificity

This kit recognizes Human AP13 in samples. No significant cross-reactivity or interference between Human AP13 and analogues was observed.

Assay Time

2.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	4℃(shading light)
Stop Solution	1 vial, 10 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Colormetric

Detection Range

125--8000pg/mL

Linearity

Samples were spiked with high concentrations of Human AP13 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	95-111	97-111	94-105
1:2	Average (%)	103	103	100
1:4	Range (%)	86-97	90-102	104
1:4	Average (%)	91	97	104
1:8	Range (%)	82-96	91-104	101-112
1:8	Average (%)	89	97	106
1:16	Range (%)	85-97	89-105	97-115
1:16	Average (%)	91	96	105

Method Type

Competitive

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human AP13 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human AP13 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	426.70	1074.70	3419.80	465.10	1177.90	3157.70
Standard deviation	28.20	51.60	143.60	31.60	56.50	132.60
C V (%)	6.61	4.80	4.20	6.79	4.80	4.20

Recovery

The recovery of Human AP13 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	92-103	98
EDTA plasma (n=5)	93-110	101
Cell culture media (n=5)	95-108	100

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

75 pg/mL

Uniprot	Q9ULZ1(65~77aa)

Manual

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10302564	E-EL-H0458	96T	$495	Add to Cart
BHE10302564	E-EL-H0458	96T*5	$2227.50	Add to Cart
BHE10302564	E-EL-H0458	96T*10	$4207.50	Add to Cart
BHE10302564	E-EL-H0458	48T	$396	Add to Cart

Human AP13(Apelin 13) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human AP13(Apelin 13) ELISA Kit

Rate it now..!!