Human ADRA1A(Adrenergic Receptor alpha-1A ) CLIA Kit

Mfr.No.

E-CL-H0229

Antigen

ADRA1A

Alternative Names

ADRA1C ; ADRA1L1 ; ALPHA1AAR

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Description

As the RLU values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)	RLU 1	RLU 2	Average RLU	Corrected RLU
4000	57719	60443	59081	59046
2000	26594	29124	27859	27824
1000	13887	13081	13484	13449
500	6224	6986	6605	6570
250	3412	3074	3243	3208
125	1700	1462	1581	1546
62.50	750	760	755	720
0	34	36	35	--

Specificity

This kit recognizes Human ADRA1A in samples. No significant cross-reactivity or interference between Human ADRA1A and analogues was observed.

Assay Time

4.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Chemiluminescence

Detection Range

62.5--4000 pg/mL

Linearity

Samples were spiked with high concentrations of Human ADRA1A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	101-113	85-95	86-100
1:2	Average (%)	107	90	93
1:4	Range (%)	97-112	96-113	90-106
1:4	Average (%)	104	103	97
1:8	Range (%)	89-100	96-110	88-100
1:8	Average (%)	94	101	95
1:16	Range (%)	102-117	99-112	89-106
1:16	Average (%)	110	106	96

Method Type

Sandwich

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ADRA1A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ADRA1A were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	185.44	371.17	1994.50	194.54	337.46	2101.72
Standard deviation	16.58	33.52	220.39	19.42	30.24	217.74
C V (%)	8.94	9.03	11.05	9.98	8.96	10.36

Recovery

The recovery of Human ADRA1A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	98-111	105
EDTA plasma (n=5)	88-100	95
Cell culture media (n=5)	98-114	105

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

37.5 pg/mL

Uniprot	P35348

Manual

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SKU	Supplier No.	Size	Your price
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BHE10300205	E-CL-H0229	96T*10	$5797	Add to Cart
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Human ADRA1A(Adrenergic Receptor alpha-1A ) CLIA Kit

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