ELISA Kit for Rat T-cell surface glycoprotein CD8 alpha chain

Mfr.No.

E0953r

Alternative Names

CD8 antigen 32 kDa chain ; OX-8 membrane antigen ; CD8a

Categories

Elisa

Function

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Specificity

Natural and recombinant Rat T-cell surface glycoprotein CD8 alpha chain

Subcellular Location

Membrane Single-pass type I membrane protein

Detection Range

0.156-10ng/mL

Entryname

CD8A_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	90-104%	92-99%	95-102%	89-103%
EDTA plasma(n=5)	88-96%	90-101%	87-103%	93-107%
heparin plasma(n=5)	94-102%	95-105%	98-109%	92-105%

Method Type

Sandwich

Platform

PF00047

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 4.5%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 9.5%

Protein Name

T-cell surface glycoprotein CD8 alpha chain

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	93-103
Plasma	98	91-105

Sensitivity

0.08ng/mL

Sequence

50:

MASRVICFLS ; LNLLLLDVIT ; RLQVSGQLQL ; SPKKVDAEIG ; QEVKLTCEVL ;

100:

RDTSQGCSWL ; FRNSSSELLQ ; PTFIIYVSSS ; RSKLNDILDP ; NLFSARKENN ;

150:

KYILTLSKFS ; TKNQGYYFCS ; ITSNSVMYFS ; PLVPVFQKVN ; SIITKPVTRA ;

200:

PTPVPPPTGT ; PRPLRPEACR ; PGASGSVEGM ; GLGFACDIYI ; WAPLAGICAV ;

236:

LLLSLVITLI ; CCHRNRRRVC ; KCPRPLVKPR ; PSEKFV

Sequence Length

236

String

P07725

Sub Unit

In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers.

Uniprot	P07725
Gene Name	Cd8a
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:24930
Unigene	Rn.10306

Reference	1. "Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes." Johnson P., Gagnon J., Barclay A.N., Williams A.F. EMBO J.4:2539-2545(1985) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] 2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] tissue: Thymus. 3. "Glycosylation sites identified by detection of glycosylated amino acids released from Edman degradation: the identification of Xaa-Pro-Xaa-Xaa as a motif for Thr-O-glycosylation." Gooley A.A., Classon B.J., Marschalek R., Williams K.L. Biochem. Biophys. Res. Commun.178:1194-1200(1991) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: GLYCOSYLATION AT ASN-63; THR-144; THR-148; THR-152; THR-158 AND THR-160

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SKU	Supplier No.	Size	Your price	Quantity
BHE13801920	E0953r	96T	$796		Add to Cart

ELISA Kit for Rat T-cell surface glycoprotein CD8 alpha chain

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