ELISA Kit for Human Prolyl 4-hydroxylase subunit alpha-1

Mfr.No.

E10871h

Alternative Names

Procollagen-proline ; 2-oxoglutarate-4-dioxygenase subunit alpha-1 ; P4HA

Categories

Elisa

Function

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

Specificity

Natural and recombinant Human Prolyl 4-hydroxylase subunit alpha-1

Subcellular Location

Endoplasmic reticulum lumen

Detection Range

0.625-40ng/mL

Entryname

P4HA1_HUMAN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF03171

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

Prolyl 4-hydroxylase subunit alpha-1

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sensitivity

0.31ng/mL

Sequence

50:

MIWYILIIGI ; LLPQSLAHPG ; FFTSIGQMTD ; LIHTEKDLVT ; SLKDYIKAEE ;

100:

DKLEQIKKWA ; EKLDRLTSTA ; TKDPEGFVGH ; PVNAFKLMKR ; LNTEWSELEN ;

150:

LVLKDMSDGF ; ISNLTIQRQY ; FPNDEDQVGA ; AKALLRLQDT ; YNLDTDTISK ;

200:

GNLPGVKHKS ; FLTAEDCFEL ; GKVAYTEADY ; YHTELWMEQA ; LRQLDEGEIS ;

250:

TIDKVSVLDY ; LSYAVYQQGD ; LDKALLLTKK ; LLELDPEHQR ; ANGNLKYFEY ;

300:

IMAKEKDVNK ; SASDDQSDQK ; TTPKKKGVAV ; DYLPERQKYE ; MLCRGEGIKM ;

350:

TPRRQKKLFC ; RYHDGNRNPK ; FILAPAKQED ; EWDKPRIIRF ; HDIISDAEIE ;

400:

IVKDLAKPRL ; RRATISNPIT ; GDLETVHYRI ; SKSAWLSGYE ; NPVVSRINMR ;

450:

IQDLTGLDVS ; TAEELQVANY ; GVGGQYEPHF ; DFARKDEPDA ; FKELGTGNRI ;

500:

ATWLFYMSDV ; SAGGATVFPE ; VGASVWPKKG ; TAVFWYNLFA ; SGEGDYSTRH ;

534:

AACPVLVGNK ; WVSNKWLHER ; GQEFRRPCTL ; SELE

Sequence Length

534

String

P13674

Sub Unit

Heterotetramer of two alpha-1 chains and two beta chains (the beta chain is the multi-functional PDI).

Uniprot	P13674
Gene Name	P4HA1
Immunogen Species	Homo Sapiens (Human)
Kegg	hsa:5033
Unigene	Hs.500047

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SKU	Supplier No.	Size	Your price	Quantity
BHE13808537	E10871h	96T	$736		Add to Cart

ELISA Kit for Human Prolyl 4-hydroxylase subunit alpha-1

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