15d-PGJ2(15-DeoXy-Delta12,14-prostaglandin J2) ELISA Kit

Mfr.No.

E-EL-0087

Antigen

15d-PGJ2

Background

This kit uses competitive-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with ; 15d-PGJ2 .During the reaction, 15d-PGJ2 in the sample or standard competes with a fixed amount of 15d-PGJ2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 15d-PGJ2 Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 15d-PGJ2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Description

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	O.D	Average
200	0.382 0.432	0.407
4000	0.505 0.5570000000000001	0.531
2000	0.745 0.741	0.743
1000	1.043 1.079	1.061
500	1.451 1.451	1.451
250	1.832 1.832	1.832
3.13	2.125 2.127	2.126
0	2.541 2.547	2.544

Specificity

This kit recognizes 15d-PGJ2 in samples. No significant cross-reactivity or interference between 15d-PGJ2 and analogues was observed.

Assay Time

2.5h

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	4℃(shading light)
Stop Solution	1 vial, 10 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Detection Method

Colormetric

Detection Range

3.125--200ng/mL

Linearity

Samples were spiked with high concentrations of 15d-PGJ2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	92-107	89-100	96-108
1:2	Average (%)	100	95	103
1:4	Range (%)	97-113	91-105	97
1:4	Average (%)	104	98	97
1:8	Range (%)	101-114	88-102	86-98
1:8	Average (%)	107	94	92
1:16	Range (%)	95-110	90-103	87-100
1:16	Average (%)	101	96	94

Method Type

Competitive

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level 15d-PGJ2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level 15d-PGJ2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	422.00	851.50	2917.80	453.90	842.50	3029.80
Standard deviation	28.30	40.00	116.70	26.80	43.00	160.60
C V (%)	6.71	4.70	4.00	5.90	5.10	5.30

Recovery

The recovery of 15d-PGJ2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	92-106	99
EDTA plasma (n=5)	94-107	99
Cell culture media (n=5)	93-106	100

Sample Type

Serum, Plasma, Cell supernatant

Sensitivity

1.88 ng/mL

Manual

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SKU	Supplier No.	Size	Your price
BHE10302550	E-EL-0087	96T	$495	Add to Cart
BHE10302550	E-EL-0087	96T*5	$2227.50	Add to Cart
BHE10302550	E-EL-0087	96T*10	$4207.50	Add to Cart
BHE10302550	E-EL-0087	48T	$396	Add to Cart

15d-PGJ2(15-Deoxy-Delta12,14-prostaglandin J2) ELISA Kit

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