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ELISA Kit for Pig V(D)J recombination-activating protein 1

SKU : BHE13808702

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EIAAB SCIENCE INC, WUHAN

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Mfr.No.	E1550p
Categories	Elisa
Function	Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1.
Specificity	Natural and recombinant Pig V(D)J recombination-activating protein 1
Subcellular Location	Nucleus
Detection Range	0.156-10ng/ml
Entryname	RAG1_PIG
Method Type	Sandwich
Platform	PF12940
Protein Name	V(D)J recombination-activating protein 1
Sensitivity	0.063ng/ml
Sequence	50: MAVSLPPTLG ; LSSAPDEIQH ; PHIKFSEWKF ; KLFRVRSFEK ; APEKAQTEKQ ; 100: DSSEGKPSLE ; QSPAVLDKPG ; GQKSALPQPA ; FKPHPKFLKE ; SHEDGKARDK ; 150: AIHQANLRRL ; CRICGNSFNT ; TGHKRRYPVH ; GPVDGKTQVL ; LRKKEKRATS ; 200: WPDLIAKVFR ; IDVKADVDSI ; HPTEFCHNCW ; SFMHRKFSST ; PCEVYSPRNA ; 250: AMEWHPHTLN ; CDICHIARRG ; LKRKSQQPNM ; QLSKKLKTVI ; DRARQARQRK ; 300: RRAQARISSK ; ELMKKIANCG ; QIHLSPKLLA ; VDFPAHFVKS ; ISCQICEHIL ; 350: ADPVETSCKH ; VFCRICILRC ; LKVMGSSCPS ; CHYPCFPTDL ; ESPVKSFLSI ; 400: LNTLMVKCPA ; KECNEEISLE ; KYNHHISSHK ; ESKETFVHIN ; KGGRPRQHLL ; 450: SLTRRAQKHR ; LRELKLQVKA ; FADKEEGGDV ; KSVCLTLFLL ; VLRARNEHRQ ; 500: ADELEAIMRG ; QGSGLQPAVC ; LAIRVNTFLS ; CSQYHKMYRT ; VKAITGRQIF ; 550: QPLHALRNAE ; KVLLPGYHPF ; EWQPPLKNVS ; SSTDVGIIDG ; LSGLSSSVDD ; 600: YPVDTIAKRF ; RYDSALVSAL ; MDMEEDILEG ; MRAQDLDDYL ; NGPFTVVVKE ; 650: SCDGMGDVSE ; KHGSGPVVPE ; KAVRFSFTVM ; KITIAHGSQN ; VKVFEEAKPN ; 700: SELCCKPLCL ; MLADESDHET ; LTAILSPLIA ; EREAMKSSQL ; MLEMGGILRT ; 750: FKFIFRGTGY ; DEKLVREVEG ; LEASGSVYIC ; TLCDATRLEA ; SQNLVFHSIT ; 800: RSHAENLERY ; EVWRSNPYHE ; TVDELRDRVK ; GVSAKPFIET ; VPSIDALHCD ; 850: IGNAAEFYKI ; FQLEIGEAYK ; NPHASKEERK ; RWQATLDKHL ; RKKMNLKPIM ; 900: RMNGNFARKL ; MTKETVEAVC ; ELIPSEERHE ; ALRELMDLYL ; KMKPVWRSSC ; 950: PAKECPESLC ; QYSFNSQRFA ; ELLSTKFKYR ; YEGKITNYFH ; KTLAHVPEII ; 1000: ERDGSIGAWA ; SEGNESGNKL ; FRRFRKMNAR ; QSKYYEMEDV ; LKHHWLYTSK ; 1043: YLQKFMNAHK ; AFKNSGFTIN ; LQRSSGDTLD ; LENSPESQDL ; MEF
Sequence Length	1043
String	Q867B5
Sub Unit	Homodimer. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2.

Uniprot	Q867B5
Gene Name	RAG1
Immunogen Species	Sus Scrofa (Pig)
Kegg	ssc:397506
Unigene	Ssc.16265

Reference	1. "Porcine RAG-1 gene promoter and coding region." Hatsuse H., Homma D., Hiraiwa H., Yasue H., Takagaki Y. Submitted (2002-09) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] strain: Large white. tissue: Liver.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13808702	E1550p	96T	$900		Add to Cart

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