Matrices listed below were spiked with certain level of VB12 and the recovery rates were calculated by comparing the measured value to the expected amount of VB12 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-102
95
EDTA plasma(n=5)
81-102
94
heparin plasma(n=5)
82-101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VB12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
80-96%
87-99%
94-103%
EDTA plasma(n=5)
92-101%
90-96%
92-100%
heparin plasma(n=5)
93-99%
91-99%
90-100%
Sensitivity
< 25pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
VB12 ELISA Kit allows for the in vitro quantitative determination of VB12 concentrations in serum, plasma, tissue homogenates and other biological fluids.