Matrices listed below were spiked with certain level of IL-2 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
95
EDTA plasma(n=5)
85-102
94
heparin plasma(n=5)
91-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
IL-2 ELISA Kit allows for the in vitro quantitative determination of IL-2 concentrations in serum, plasma, tissue homogenates and other biological fluids.