Rat thrombin-antithrombin complex,TAT ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08432r

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

thrombin-antithrombin complex,TAT

Recovery

The recovery of human BF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	88-101
EDTA plasma (n=4)	97	91-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	97
	Range %	89-105
1:2000	Average %	89
	Range %	85-98
1:4000	Average %	93
	Range %	90-97
1:8000	Average %	96
	Range %	89-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	TAT

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Impact of an autologous oxygenating matrix culture system on rat islet transplantation outcome A. Schaschkow et al,Biomaterials,2015 Jun;52:180-8 Pretreatment with rivaroxaban attenuates stroke severity in rats by a dual antithrombotic and anti-inflammatory mechanism. Dittmeier M.et al,Thromb Haemost.,115(4):835-43 Therapeutic targeting of extracellular DNA improves the outcome of intestinal ischemic reperfusion injury in neonatal rats Boettcher M.et al,Sci Rep,Nov 13 Assessment of Thrombotic Risk in Atrial Fibrillation with Ultrasound Molecular Imaging of P-Selectin Jing Y.et al,Thromb Haemost,/

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