Rat procollagen type III (HPCIII) ELISA Kit

Mfr.No.

CSB-E15810r

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

procollagen type III (HPCIII), procollagen type 3 ,HPC3, procollagen type III ,HPCIII procollagen type III ,HPCIII, procollagen type III (HPCIII), procollagen type 3 ,HPC3, procollagen type III ,HPCIII procollagen type III ,HPCIII

Recovery

The recovery of mouse CYP2E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	92	88-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CYP2E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	96
	Range %	92-100
1:4	Average %	91
	Range %	85-95
1:8	Average %	104
	Range %	100-113

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2500	2.482	2.658	2.570	2.384
1250	1.968	2.076	2.022	1.836
625	1.418	1.503	1.461	1.275
312	0.833	0.857	0.845	0.659
156	0.555	0.521	0.538	0.352
78	0.315	0.321	0.318	0.132
39	0.235	0.244	0.240	0.054
0	0.183	0.188	0.186	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	HPCIII

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508520	CSB-E15810r	96T	$790	Add to Cart
BHE10508520	CSB-E15810r	5x96T	$3792	Add to Cart
BHE10508520	CSB-E15810r	10x96T	$7505	Add to Cart

Rat procollagen type III (HPCIII) ELISA Kit

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