Matrices listed below were spiked with certain level of NT-Pro-ANP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-Pro-ANP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
97
EDTA plasma(n=5)
87-104
94
heparin plasma(n=5)
85-102
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-Pro-ANP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
NT-Pro-ANP ELISA Kit allows for the in vitro quantitative determination of NT-Pro-ANP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat NT-Pro-ANP(N-Terminal Pro-Atrial Natriuretic Peptide) ELISA Kit