Rat Interferon Gamma - IFN-G ELISA Kit

Mfr.No.

E0103Ra

Sensitivity

0.51ng/L

Detection Range

1-380ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Interferon γ (also known as IFN-γ) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IFN G;IFN gamma;IFNG;IFN-gamma;Interferon gamma

Molecular Weight

17918

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0103Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	119.1	7.14	6
Intra-Assay	2	18	58.6	3.21	5.5
Intra-Assay	3	18	15.3	0.87	5.7

Background

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Protocol

These standard curves of E0103Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
200ng/L	2.265	2.174	2.127
	2.083
100ng/L	1.545	1.65	1.603
	1.754
50ng/L	1.14	1.071	1.024
	1.002
25ng/L	0.675	0.719	0.672
	0.764
12.5ng/L	0.441	0.431	0.384
	0.422
0ng/L	0.047	0.047	0
	0.048

Gene ID	25712
UniProt ID	P01581

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111421	E0103Ra	48T	$320		Add to Cart
BHE12111421	E0103Ra	96T	$458		Add to Cart

Rat Interferon Gamma - IFN-G ELISA Kit

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