Matrices listed below were spiked with certain level of IgE and the recovery rates were calculated by comparing the measured value to the expected amount of IgE in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-97
90
EDTA plasma(n=5)
85-103
97
heparin plasma(n=5)
91-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-105%
85-99%
86-94%
EDTA plasma(n=5)
83-97%
84-96%
82-101%
heparin plasma(n=5)
80-99%
80-98%
83-98%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
Antigen
IgE
Applications
ELISA
Applications Notes
IgE Elisa kit allows for the in vitro quantitative determination of IgE concentrations in serum, plasma, tissue homogenates and other biological fluids.