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Home / ELISA Kit

Mouse Tumor necrosis factor ligand superfamily member 13 (TNFSF13) ELISA Kit

SKU : BHE13705277

Tumor necrosis factor ligand superfamily member 13 is a member of the tumor necrosis factor (TNF) ligand family. Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages.
This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

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Mfr.No.	KTE70116
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Tumor necrosis factor ligand superfamily member 13 (TNFSF13) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF13.
Alternative Names	TNFSF13; APRIL; CD256; TALL2; TRDL-1; UNQ383/PRO715; ligand; TNF- and APOL-related leukocyte expressed ligand 2; a proliferation inducing ligand; tumor necrosis factor (ligand) superfamily member 13 transcript
Detection Method	Colorimetric
Kit Components	Mouse Tumor necrosis factor ligand superfamily member 13 microplate Mouse Tumor necrosis factor ligand superfamily member 13 standard Mouse Tumor necrosis factor ligand superfamily member 13 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Tumor necrosis factor ligand superfamily member 13 (TNFSF13) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFSF13. No significant cross-reactivity or interference between Mouse TNFSF13 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Tumor necrosis factor ligand superfamily member 13 is a member of the tumor necrosis factor (TNF) ligand family. Expressed at high levels in transformed cell lines, cancers of colon, thyroid, lymphoid tissues and specifically expressed in monocytes and macrophages. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Notes	This Mouse Tumor necrosis factor ligand superfamily member 13 (TNFSF13) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF13 in samples. An antibody specific for TNFSF13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	69583

Applications	ELISA
Applications Notes	This Mouse Tumor necrosis factor ligand superfamily member 13 (TNFSF13) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF13 in samples. An antibody specific for TNFSF13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705277	KTE70116	48 T	$339		Add to Cart
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BHE13705277	KTE70116	96 Tx50	$24569		Add to Cart

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