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Home / ELISA Kit

Mouse Tubulointerstitial nephritis antigen-like (TINAGL1) ELISA Kit

SKU : BHE13705385

Tubulointerstitial nephritis antigen-like 1 has been cloned from mouse adrenocortical cells and is known to be closely associated with zonal differentiation of adrenocortical cells. In cell culture systems, TINAGL1 is a matricellular protein that interacts with both structural matrix proteins and cell surface receptors. However, the physiological roles of TINAGL1 and regulation of its expression are still not clearly understood.
In the present study, the expression and localization of TINAGL1 in peri-implantation mouse embryos was examined. During preimplantation, the expression of both Tinagl1 mRNA and TINAGL1 protein was increased just prior to implantation. In blastocysts, TINAGL1 expression was localized to the trophectoderm.

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Mfr.No.	KTE70225
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Tubulointerstitial nephritis antigen-like (TINAGL1) ELISA Kit employs a two-site sandwich ELISA to quantitate TINAGL1.
Alternative Names	TINAGL1; ARG1; LCN7; LIECG3; TINAGRP; P3ECSL; TINAG-like 1; androgen-regulated gene 1; glucocorticoid-inducible protein; lipocalin 7; oxidized-LDL responsive gene 2; tubulointerstitial nephritis antigen-related pr
Detection Method	Colorimetric
Kit Components	Mouse Tubulointerstitial nephritis antigen-like microplate Mouse Tubulointerstitial nephritis antigen-like standard Mouse Tubulointerstitial nephritis antigen-like detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Tubulointerstitial nephritis antigen-like (TINAGL1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TINAGL1. No significant cross-reactivity or interference between Mouse TINAGL1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Tubulointerstitial nephritis antigen-like 1 has been cloned from mouse adrenocortical cells and is known to be closely associated with zonal differentiation of adrenocortical cells. In cell culture systems, TINAGL1 is a matricellular protein that interacts with both structural matrix proteins and cell surface receptors. However, the physiological roles of TINAGL1 and regulation of its expression are still not clearly understood. In the present study, the expression and localization of TINAGL1 in peri-implantation mouse embryos was examined. During preimplantation, the expression of both Tinagl1 mRNA and TINAGL1 protein was increased just prior to implantation. In blastocysts, TINAGL1 expression was localized to the trophectoderm.
Notes	This Mouse Tubulointerstitial nephritis antigen-like (TINAGL1) ELISA Kit employs a two-site sandwich ELISA to quantitate TINAGL1 in samples. An antibody specific for TINAGL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTINAGL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TINAGL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TINAGL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	94242

Applications	ELISA
Applications Notes	This Mouse Tubulointerstitial nephritis antigen-like (TINAGL1) ELISA Kit employs a two-site sandwich ELISA to quantitate TINAGL1 in samples. An antibody specific for TINAGL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTINAGL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TINAGL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TINAGL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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BHE13705385	KTE70225	48 T	$339		Add to Cart
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