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Home / ELISA Kit

Mouse Trinucleotide repeat-containing gene 18 protein (TNRC18) ELISA Kit

SKU : BHE13705251

Eleven novel TPGs were discovered: DCP1A (decapping enzyme homologue A), (trinucleotide repeat containing 18), LAMC3 (laminin, g-3), NEBL (nebulette), NRIP3 (nuclear receptor interacting protein 3), C2CD3 (C2 calciumdependent domain containing 3), UBE4A (ubiquitination factor from the gel and subjected to DNA sequence analyses to obtain the patient-specific fusion sequences. The DCP1A gene encodes a homologue of the DCP1 decapping enzyme, involved in mRNA degradation.15 The protein localizes in the mRNA processing body (P-body) that regulates degradation and abundance of RNA molecules (RNA decay). For TNRC18, no function is known. LAMC3 is a nonbasement membrane-associate filamentous protein that is downregulated or deleted in carcinomas.

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Mfr.No.	KTE70089
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Trinucleotide repeat-containing gene 18 protein (TNRC18) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC18.
Alternative Names	TNRC18; TNRC18;
Detection Method	Colorimetric
Kit Components	Mouse Trinucleotide repeat-containing gene 18 protein microplate Mouse Trinucleotide repeat-containing gene 18 protein standard Mouse Trinucleotide repeat-containing gene 18 protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Trinucleotide repeat-containing gene 18 protein (TNRC18) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNRC18. No significant cross-reactivity or interference between Mouse TNRC18 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Eleven novel TPGs were discovered: DCP1A (decapping enzyme homologue A), (trinucleotide repeat containing 18), LAMC3 (laminin, g-3), NEBL (nebulette), NRIP3 (nuclear receptor interacting protein 3), C2CD3 (C2 calciumdependent domain containing 3), UBE4A (ubiquitination factor from the gel and subjected to DNA sequence analyses to obtain the patient-specific fusion sequences. The DCP1A gene encodes a homologue of the DCP1 decapping enzyme, involved in mRNA degradation.15 The protein localizes in the mRNA processing body (P-body) that regulates degradation and abundance of RNA molecules (RNA decay). For TNRC18, no function is known. LAMC3 is a nonbasement membrane-associate filamentous protein that is downregulated or deleted in carcinomas.
Notes	This Mouse Trinucleotide repeat-containing gene 18 protein (TNRC18) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC18 in samples. An antibody specific for TNRC18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNRC18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNRC18 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNRC18 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	231861

Applications	ELISA
Applications Notes	This Mouse Trinucleotide repeat-containing gene 18 protein (TNRC18) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC18 in samples. An antibody specific for TNRC18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNRC18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNRC18 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNRC18 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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