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Home / ELISA Kit

Mouse Transketolase-like protein 1 (TKTL1) ELISA Kit

SKU : BHE13705376

Transketolase (TKT;) is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. As part of a systematic search for differentially expressed genes, Coy et al. (1996) isolated a novel transketolase-related gene, TKTL1, which they called TKR. They isolated transcripts encoding tissue-specific protein isoforms. Comparison with known transketolases demonstrated a TKR-specific deletion mutating 1 thiamine-binding site.
Genomic sequencing of the TKR gene by Coy et al. (1996) revealed the presence of a pseudoexon, as well as the acquisition of a tissue-specific spliced exon, compared to the TKT gene, which maps to 3p14.3.

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Mfr.No.	KTE70216
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Transketolase-like protein 1 (TKTL1) ELISA Kit employs a two-site sandwich ELISA to quantitate TKTL1.
Alternative Names	TKTL1; LL0XNC01-14B7.1; TKR; TKT2; transketolase-2; transketolase-related protein
Detection Method	Colorimetric
Kit Components	Mouse Transketolase-like protein 1 microplate Mouse Transketolase-like protein 1 standard Mouse Transketolase-like protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Transketolase-like protein 1 (TKTL1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TKTL1. No significant cross-reactivity or interference between Mouse TKTL1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Transketolase (TKT;) is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. As part of a systematic search for differentially expressed genes, Coy et al. (1996) isolated a novel transketolase-related gene, TKTL1, which they called TKR. They isolated transcripts encoding tissue-specific protein isoforms. Comparison with known transketolases demonstrated a TKR-specific deletion mutating 1 thiamine-binding site. Genomic sequencing of the TKR gene by Coy et al. (1996) revealed the presence of a pseudoexon, as well as the acquisition of a tissue-specific spliced exon, compared to the TKT gene, which maps to 3p14.3.
Notes	This Mouse Transketolase-like protein 1 (TKTL1) ELISA Kit employs a two-site sandwich ELISA to quantitate TKTL1 in samples. An antibody specific for TKTL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTKTL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TKTL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TKTL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	83553

Applications	ELISA
Applications Notes	This Mouse Transketolase-like protein 1 (TKTL1) ELISA Kit employs a two-site sandwich ELISA to quantitate TKTL1 in samples. An antibody specific for TKTL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTKTL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TKTL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TKTL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705376	KTE70216	48 T	$339		Add to Cart
BHE13705376	KTE70216	96 T	$589		Add to Cart
BHE13705376	KTE70216	96 Tx5	$2629		Add to Cart
BHE13705376	KTE70216	96 Tx50	$24569		Add to Cart

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