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Home / ELISA Kit

Mouse TIMELESS-interacting protein (TIPIN) ELISA Kit

SKU : BHE13705382

TIPIN associated with the components of the replicative helicase in human cell lines and protected cells against genotoxic agents. TIPIN was required for efficient cell cycle arrest in response to DNA damage, and depletion of TIPIN rendered cells sensitive to ionizing radiation as well as replication stress.It shares 72% amino acid identity with mouse Tipin, with the greatest divergence in the C termini. coexpression of Tim and Tipin in early embryonic and extraembryonic tissues, although Tipin was more widely expressed. After embryonic day 14.5, both Tim and Tipin were abundantly expressed in thymus, liver, gastrointestinal tract, and ventricular zone of brain, and Tipin was also expressed in lung. In adult mouse brain, Tipin was expressed in thalamic and cortical regions, with lower expression in suprachiasmatic nuclei.

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Mfr.No.	KTE70222
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse TIMELESS-interacting protein (TIPIN) ELISA Kit employs a two-site sandwich ELISA to quantitate TIPIN.
Alternative Names	TIPIN; FLJ20516; timeless-interacting protein
Detection Method	Colorimetric
Kit Components	Mouse TIMELESS-interacting protein microplate Mouse TIMELESS-interacting protein standard Mouse TIMELESS-interacting protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse TIMELESS-interacting protein (TIPIN) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TIPIN. No significant cross-reactivity or interference between Mouse TIPIN and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TIPIN associated with the components of the replicative helicase in human cell lines and protected cells against genotoxic agents. TIPIN was required for efficient cell cycle arrest in response to DNA damage, and depletion of TIPIN rendered cells sensitive to ionizing radiation as well as replication stress.It shares 72% amino acid identity with mouse Tipin, with the greatest divergence in the C termini. coexpression of Tim and Tipin in early embryonic and extraembryonic tissues, although Tipin was more widely expressed. After embryonic day 14.5, both Tim and Tipin were abundantly expressed in thymus, liver, gastrointestinal tract, and ventricular zone of brain, and Tipin was also expressed in lung. In adult mouse brain, Tipin was expressed in thalamic and cortical regions, with lower expression in suprachiasmatic nuclei.
Notes	This Mouse TIMELESS-interacting protein (TIPIN) ELISA Kit employs a two-site sandwich ELISA to quantitate TIPIN in samples. An antibody specific for TIPIN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIPIN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIPIN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIPIN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	66131

Applications	ELISA
Applications Notes	This Mouse TIMELESS-interacting protein (TIPIN) ELISA Kit employs a two-site sandwich ELISA to quantitate TIPIN in samples. An antibody specific for TIPIN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIPIN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIPIN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIPIN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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