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Home / ELISA Kit

Mouse Spectrin alpha chain, brain (SPTAN1) ELISA Kit

SKU : BHE13705409

Alpha-Fodrin is an intracellular, actin-binding, organ-specific protein of the cytoskeleton. The network of actin and fodrin situated below the plasma membrane of secretorial cells, is important for the alignment of secretorial vesicles to the plasma membrane during secretorial processes. During apoptosis the alpha-fodrin dimer is cleaved into a 120 kDa breakdown product, which is found abundantly in the salivary gland. This proteolysis of fodrin may be a consequence of protease activation during apoptosis. The cleavage product of 120 kDa alpha-fodrin was found to be an important autoantigen in the pathogenesis of organ-specific autoimmune esponse. Clinical studies have shown, that in patients with Sjögren Syndrome alpha-fodrin is involved in the stimulation of peripheral blood T-cells.

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Mfr.No.	KTE70249
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Spectrin alpha chain, brain (SPTAN1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTAN1.
Alternative Names	SPTAN1; (ALPHA)II-SPECTRIN; FLJ44613; NEAS; non-erythrocytic spectrin alpha
Detection Method	Colorimetric
Kit Components	Mouse Spectrin alpha chain, brain microplate Mouse Spectrin alpha chain, brain standard Mouse Spectrin alpha chain, brain detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Spectrin alpha chain, brain (SPTAN1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPTAN1. No significant cross-reactivity or interference between Mouse SPTAN1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Alpha-Fodrin is an intracellular, actin-binding, organ-specific protein of the cytoskeleton. The network of actin and fodrin situated below the plasma membrane of secretorial cells, is important for the alignment of secretorial vesicles to the plasma membrane during secretorial processes. During apoptosis the alpha-fodrin dimer is cleaved into a 120 kDa breakdown product, which is found abundantly in the salivary gland. This proteolysis of fodrin may be a consequence of protease activation during apoptosis. The cleavage product of 120 kDa alpha-fodrin was found to be an important autoantigen in the pathogenesis of organ-specific autoimmune esponse. Clinical studies have shown, that in patients with Sjögren Syndrome alpha-fodrin is involved in the stimulation of peripheral blood T-cells.
Notes	This Mouse Spectrin alpha chain, brain (SPTAN1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTAN1 in samples. An antibody specific for SPTAN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPTAN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTAN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTAN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Mouse Spectrin alpha chain, brain (SPTAN1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTAN1 in samples. An antibody specific for SPTAN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPTAN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTAN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTAN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705409	KTE70249	48 T	$339		Add to Cart
BHE13705409	KTE70249	96 T	$589		Add to Cart
BHE13705409	KTE70249	96 Tx5	$2629		Add to Cart
BHE13705409	KTE70249	96 Tx50	$24569		Add to Cart

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