Matrices listed below were spiked with certain level of RG2 and the recovery rates were calculated by comparing the measured value to the expected amount of RG2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-104
99
EDTA plasma(n=5)
88-98
92
heparin plasma(n=5)
92-100
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RG2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-105%
88-105%
85-101%
EDTA plasma(n=5)
83-99%
83-101%
88-101%
heparin plasma(n=5)
82-100%
80-99%
81-96%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q61878
Applications
ELISA
Applications Notes
RG2 ELISA Kit allows for the in vitro quantitative determination of RG2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse RG2(Bone marrow proteoglycan) ELISA Kit