Mouse pyridinoline (PYD) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E15010m

Alternative Names

Pyridinoline crosslinks,PYD

Reactivity

Mouse

Detection Range

50 ng/mL-800 ng/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of mouse HA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	97	93-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	89
	Range %	82-99
1:200	Average %	93
	Range %	89-101
1:400	Average %	102
	Range %	98-106
1:800	Average %	97
	Range %	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

50 ng/mL

Limit Of Detection

450 nm

Antigen	Pyridinoline crosslinks,PYD

Applications

ELISA

Research Area

PYD

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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