Mouse pancreatic alpha amylase (AMY2A)ELISA kit

Mfr.No.

CSB-EL001689MO

Sensitivity

2.3 mU/mL

Detection Range

9.38 mU/mL-600 mU/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

pancreatic alpha amylase (AMY2A), AMY2, AMY2B, PA, 1,4-alpha-D-glucan glucanohydrolase, alpha-amylase, amylase, pancreatic, alpha-2A, found in the pancreas, glycogenase, pancreatic amylase 2A, pancreatic amylase alpha 2A

Recovery

The recovery of human GGT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-98
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human GGT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	93
	Range %	89-97
1:4	Average %	88
	Range %	84-92
1:8	Average %	104
	Range %	100-112

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
50	1.991	2.069	2.030	1.846
25	1.438	1.494	1.466	1.282
12.5	0.996	1.032	1.014	0.830
6.25	0.720	0.711	0.716	0.532
3.12	0.484	0.477	0.481	0.297
1.56	0.348	0.338	0.343	0.159
0.78	0.249	0.265	0.257	0.073
0	0.181	0.186	0.184	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P00688

Applications	ELISA
Research Area	AMY2A

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506404	CSB-EL001689MO	96T	$695	Add to Cart
BHE10506404	CSB-EL001689MO	5x96T	$3336	Add to Cart
BHE10506404	CSB-EL001689MO	10x96T	$6602.50	Add to Cart

Mouse pancreatic alpha amylase (AMY2A)ELISA kit

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