Mouse Ovalbumin Specific IgG(OVA sIgG) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14990m

Sensitivity

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Detection Range

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Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

Ovalbumin Specific IgG(OVA sIgG)

Recovery

The recovery of mouse IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	89	84-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	106
	Range %	101-113
1:2	Average %	97
	Range %	92-103
1:4	Average %	90
	Range %	85-94
1:8	Average %	94
	Range %	90-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	OVA sIgG

Storage	Store at 2-8°C. Please refer to protocol.

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