Mouse FRAP(FK506 Binding Protein 12 Rapamycin Associated Protein) ELISA Kit

Mfr.No.

ELK8589

Sensitivity

0.062 ng/mL

Detection Range

0.16-10 ng/mL

Reactivity

Mouse

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

FRAP1; MTOR; FRAP2; MTOR; RAFT1; RAPT1; Mammalian Target Of Rapamycin; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin and FKBP12 target 1

Recovery

Matrices listed below were spiked with certain level of recombinant FRAP and the recovery rates were calculated by comparing the measured value to the expected amount of FRAP in samples.

Matrix	Recovery range	Average
serum(n=5)	80-90%	85%
EDTA plasma(n=5)	84-106%	100%
Heparin plasma(n=5)	87-93%	90%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FRAP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-96%	96-105%	81-93%	93-102%
EDTA plasma(n=5)	83-97%	84-99%	95-102%	88-103%
Heparin plasma(n=5)	86-95%	91-106%	87-100%	95-101%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
10.00	2.054	1.967
5.00	1.761	1.674
2.50	1.028	0.941
1.25	0.889	0.802
0.63	0.549	0.462
0.32	0.387	0.300
0.16	0.100	0.013
0.00	0.087	0.000

Limit Of Detection

10 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FRAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FRAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FRAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FRAP in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	Q9JLN9

Applications	ELISA
Research Area	Tumor immunity;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15205776	ELK8589	48T	$320		Add to Cart
BHE15205776	ELK8589	96T	$458		Add to Cart

Mouse FRAP(FK506 Binding Protein 12 Rapamycin Associated Protein) ELISA Kit

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