Mouse E3 Ubiquitin-protein Ligase Smurf1

Mfr.No.

E2036Mo

Alternative Names

E3 ubiquitin-protein ligase SMURF1;HECT-type E3 ubiquitin transferase SMURF1;SMAD ubiquitination regulatory factor 1;SMAD-specific E3 ubiquitin-protein ligase 1;SMURF 1;SMURF1

Reactivity

Mouse

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

83356

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.56ng/L

Background

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway (By similarity). Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed: 23087404). Plays a role in dendrite formation by melanocytes (By similarity).

Gene ID	75788
UniProt ID	Q9CUN6
Antigen	SMURF1

Research Area

Cell Biology

Protocol

These standard curves of E2036Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.08	2.066	2.007
	2.051
1200ng/L	1.095	0.996	0.938
	0.898
600ng/L	0.556	0.57	0.511
	0.584
300ng/L	0.212	0.237	0.178
	0.262
150ng/L	0.12	0.139	0.08
	0.158
0ng/L	0.056	0.059	0
	0.061

Precision

We measured random samples of E2036Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1861	31	1.7
Intra-Assay	2	18	708	41.16	5.8
Intra-Assay	3	18	1412.2	67.27	4.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109847	E2036Mo	48T	$320		Add to Cart
BHE12109847	E2036Mo	96T	$458		Add to Cart

Mouse E3 Ubiquitin-protein Ligase Smurf1 - SMURF1 ELISA Kit

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