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Home / ELISA Kit

Mouse Antigen peptide transporter 1 (TAP1) ELISA Kit

SKU : BHE13705500

The membrane-associated protein encoded by Tap1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This protein forms a heterodimer with Tap2 that transports short peptides from the cytosol into the endoplasmic reticulum lumen. Mutations in the human gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for Tap1.

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Mfr.No.	KTE70340
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Antigen peptide transporter 1 (TAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAP1.
Alternative Names	TAP1; DAAP-57C1.5; ABC17; ABCB2; APT1; D6S114E; FLJ26666; FLJ41500; PSF1; RING4; TAP1*0102N; TAP1N; ABC transporter; MHC 1; ATP-binding cassette; sub-family B (MDR/TAP); member 2; antigen peptide transporte
Detection Method	Colorimetric
Kit Components	Mouse Antigen peptide transporter 1 microplate Mouse Antigen peptide transporter 1 standard Mouse Antigen peptide transporter 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Antigen peptide transporter 1 (TAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TAP1. No significant cross-reactivity or interference between Mouse TAP1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The membrane-associated protein encoded by Tap1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This protein forms a heterodimer with Tap2 that transports short peptides from the cytosol into the endoplasmic reticulum lumen. Mutations in the human gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for Tap1.
Notes	This Mouse Antigen peptide transporter 1 (TAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAP1 in samples. An antibody specific for TAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	21354

Applications	ELISA
Applications Notes	This Mouse Antigen peptide transporter 1 (TAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAP1 in samples. An antibody specific for TAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705500	KTE70340	48 T	$339		Add to Cart
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